Search results for "TR146 cells"

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The membrane-associated MUC1 improves adhesion of salivary MUC5B on buccal cells. Application to development of an in vitro cellular model of oral ep…

2015

Objectives: The mucosal pellicle is a thin layer of salivary proteins, mostly MUC5B mucins, anchored to epithelial oral cells. This pellicle is involved in protection of oral mucosae against abrasion, pathogenic microorganisms or chemical xenobiotics. The present study aimed at studying the involvement of MUC1 in mucosal pellicle formation and more specifically in salivary MUC5B binding using a cell-based model of oral epithelium. DESIGN: MUC1 mRNAs were not detected in TR146 cells, and therefore a stable cell line named TR146/MUC1 expressing this protein was developed by transfection. TR146 and TR146/MUC1 were incubated with human saliva in order to evaluate retention of MUC5B by epithelia…

0301 basic medicineSaliva[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionEpithelium0302 clinical medicineimmunocytochemistryTR146 cellsDental PellicleOral mucosa[ SDV.MHEP.CHI ] Life Sciences [q-bio]/Human health and pathology/SurgeryMUC1Microscopy ConfocalReverse Transcriptase Polymerase Chain ReactionGeneral MedicineTransfectionImmunohistochemistryMucin-5Bmedicine.anatomical_structuremucosal pelliclescanning electron microscopyImmunoblotting[SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/SurgeryBiologyIn Vitro TechniquesTransfectionMicrobiologyCell Line03 medical and health sciences[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathologymedicineCell AdhesionHumansSalivary Proteins and PeptidesSalivaGeneral Dentistryoral mucosaMucinMucin-1Mouth Mucosa030206 dentistryCell BiologymucinsMolecular biologyIn vitroEpithelium030104 developmental biologyOtorhinolaryngologyCell cultureMicroscopy Electron Scanning[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology

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